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For these early results in Xenopus to be reproduced in mammals took nearly 40 years in sheep. A very important feature of these first successful mammalian nuclear transfer in sheep was the use of unfertilised eggs, as was actually used in amphibia. Earlier work with mice used fertilised eggs. Although fertilised eggs can be used , synchronisation between nucleus and egg is harder to achieve than with the use of unfertilised eggs. A very elegant and important experiment that confirmed the general principle that cell differentiation proceeds with the retention of a complete set of genes was carried out using nuclei with a rearranged genome from mature mouse B or T donor cells . In the course of time, somatic cell nuclear transfer to eggs has been successful in the eggs of mice and other mammals . In each species there seem to be some technical requirements which have to be identified and overcome. (en) |
